General description

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Reported to upregulate neoangiogenesis in carcinogenesis of the uterine cortex. Tryptase is also an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Native tryptase from human lung. Tryptase is a serine protease stored in mast cell granules. A tetrameric enzyme composed of four non-covalently bound subunits. Each subunit is reported to contain one active site. Tryptase is an important mediator of allergic reactions that activates G-protein-coupled receptors through proteolysis, thus amplifying histamine release.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Benitez-Bribiesca, L., et al. 2001. J. Histochem. Cytochem.49, 1061.He, S., et al. 1998. J. Pharmacol. Exp. Ther. 286, 289.Molino, M., et al. 1997. J. Biol. Chem. 272, 4043.Schwartz, L.B., and Bradford, T.R. 1986. J. Biol. Chem. 261, 7372.

Packaging

25 µg in Plastic ampoule

Physical form

In 1 M NaCl, 50 mM NaOAc, 50 µM heparin, pH 5.0.

Preparation Note

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Unit Definition

One unit is defined as the amount of enzyme that gives a change of 1 absorbance per min at 410 nm using N-benzoyl-Phe-Val-Arg-pNA as a substrate in 30 min at 25°C.

Warning

Toxicity: Irritant (B)